Gasp will take up residence in the Eastgate Shopping centre, creating an art gallery and three studios for emerging artists.
It will then work with local creatives and young people to bring artworks and events to the city.
The organisation has previously transformed unused spaces in Gloucester into cultural and communal experiences.
Its co-founder and director Hannah Thomson said the feedback from this showed “residents want to see more contemporary art exhibitions in the city, and have more opportunities to take part in creative activities”.
“Visitors were delighted to see the creative use of empty shops that have long stood empty,” she added.